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United States Department of Agriculture

Agricultural Research Service

Title: Whole Genome Mapping in a Wheat Doubled Haploid Population Using SSRs and TRAPS and the Identification of QTL for Agronomic Traits

Authors
item Chu, Chenggen - NORTH DAKOTA STATE UNIV.
item Xu, Steven
item Friesen, Timothy
item Faris, Justin

Submitted to: Molecular Breeding
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: February 21, 2008
Publication Date: March 4, 2008
Citation: Chu, C., Xu, S.S., Friesen, T.L., Faris, J.D. 2008. Whole Genome Mapping in a Wheat Doubled Haploid Population Using SSRs and TRAPS and the Identification of QTL for Agronomic Traits. Molecular Breeding. 22:251-266

Interpretive Summary: Quantitative trait loci (QTL) analysis allows the identification of genomic regions associated with quantitative traits, which provides an estimation of the number and chromosomal location of genes involved and leads to the identification of molecular markers suitable for marker-assisted selection (MAS). In this research, we used the wheat corn method to develop a doubled haploid (DH) mapping population derived from the synthesized common wheat line TA4152-60 and the North Dakota hard red spring wheat breeding line ND495. The population consisted of 213 lines, and a subset of 120 lines was randomly selected and used to construct linkage maps of all 21 chromosomes. The maps consisted of 632 markers including 629 molecular and 3 phenotypic, and spanned 3,811.5 cM with an average density of one marker per 6.03 cM. Chromosome end-specific markers allow us to define the ends of seven linkage groups. Analysis revealed major QTLs associated with the traits of days to heading, plant height, coleoptile color, and spike characteristics. The DH population and genetic map will be a useful tool for the identification of disease resistance QTL and agronomically important loci, and will aid in the identification and development of markers for MAS.

Technical Abstract: Quantitative trait loci (QTL) analysis allows the identification of genomic regions associated with quantitative traits, which provides an estimation of the number and chromosomal location of genes involved and leads to the identification of molecular markers suitable for marker-assisted selection (MAS). In this research, we used the wheat maize method to develop a doubled haploid (DH) population derived from the synthetic hexaploid wheat line TA4152-60 and the North Dakota hard red spring wheat line ND495. The population consisted of 213 lines, and a subset of 120 lines was randomly selected and used to construct linkage maps of all 21 chromosomes. The maps consisted of 632 markers including 410 SSRs, 218 TRAPs, 1 RFLP, and 3 phenotypic markers, and spanned 3,811.5 cM with an average density of one marker per 6.03 cM. Telomere sequence-based TRAPs allow us to define the ends of seven linkage groups. Analysis revealed major QTLs associated with the traits of days to heading on chromosomes 5A and 5B, plant height on chromosomes 4D and 5A, coleoptile color on chromosomes 7A and 7D, and spike characteristics on chromosomes 3D, 4A, 4D, 5A and 5B. The DH population and genetic map will be a useful tool for the identification of disease resistance QTL and agronomically important loci, and will aid in the identification and development of markers for MAS.

Last Modified: 8/29/2014
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