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United States Department of Agriculture

Agricultural Research Service

Research Project: IMPROVING GREAT LAKES AQUACULTURE PRODUCTION Title: Developing Genetically Defined Yellow Perch (Perca flavescens) Broodstocks

Authors
item Rosauer, D - GREAT LAKES WATER INST
item Grzybowski, M - GREAT LAKES WATER INST
item Binkowski, F - GREAT LAKES WATER INST
item Shepherd, Brian
item Goetz, F - GREAT LAKES WATER INST

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: July 18, 2007
Publication Date: N/A

Technical Abstract: Yellow Perch are an ecologically and economically important species common in Midwest commercial aquaculture. Broodstocks are traditionally derived from wild populations locally accessible to the producer. This can lead to inconsistencies in the success of producers within and between regions. Successful aquaculture depends on local stocks possessing multiple desirable traits including: fast growth, resistance to disease, and high fecundity. To breed for these traits, it is necessary to have a genetically defined broodstock in which the genetic variability is known. We have initiated a program to establish genetically defined yellow perch broodstocks for commercial perch aquaculture. To establish these broodstocks, several steps are being taken including 1) genetic analysis of various wild perch populations throughout North America; 2) spawning from desirable populations to obtain F1 broodstocks; 3) performance trials; and 4) selective breeding. 1. Genetic Analysis: Fin clips were obtained from 12 wild populations ranging from the upper Midwest to the Atlantic Coast. Analysis was completed using published and newly derived microsatellite markers (Figure 1: not shown). 2. Spawning to produce F1 generations: From the microsatellite results we chose the Perquimans River (North Carolina, Atlantic Coast), Choptank River (Maryland, East Coast), and Lake Winnebago (Wisconsin, upper Midwest) to obtain gametes for F1 generations (14-34 pairwise crosses of wild adults form each population). 3. Performance trial: The performance trial consists of 4 replicate tanks of 650 juveniles/tank from each population raised under identical conditions in flow-through tanks until market size (~14 months). After obtaining market size, the top performers (20%) within each population will be crossed to produce a F2 generation for future selection. Supported by NOAA Sea Grant and ARS/USDA

Last Modified: 10/22/2014