ARS | Publication request: Sequences of Wolbachia wsp genes reveal multiple infection of individual northern corn rootworms (Diabrotica barberi) by several Wolbachia strains

Submitted to: Genbank
Publication Type: Other
Publication Acceptance Date: October 22, 2007
Publication Date: October 27, 2007
Citation: Roehrdanz, R.L. 2007. Sequences of Wolbachia wsp genes reveal multiple infection of individual northern corn rootworms (Diabrotica barberi) by several Wolbachia strains. Genbank. Accession numbers: EU188677-EU188725.

Technical Abstract: Northern corn rootworm (Diabrotica barberi)(NCR) populations in the USA are infected with at least 4 strains of the endosymbiont, Wolbachia. NCR from eastern Illinois to Pennsylvania appear to harbor at least 4 different strains designated wBar1, wBar3, wBar4, and wBar5. NCR from central Illinois appears to be singly infected with a strain wBar2. NCR from western Illinois through the Great Plains are uninfected. Multiple infection was detected by sequencing multiple clones of the Wolbachia wsp gene from the same insect. Five clades are indicated by sequence comparison, length, and Tsp509I restriction patterns. The segments range from 582 to 603 bp. The wsp gene segment is composed of 4 hypervariable regions (HV) interspersed by more conserved regions. Different Wolbachia strains may have alternative mixes of these HV regions. wBar1 and wBar2 have completely different HVs. wBar3 is defined by a 3rd complete set of HVs while wBar4 appears to be unique for HV2-HV4. The latter was found with both wBAr1 and wBar2 HV1. A single alternate HV2 signals a possible 5th Wolbachia strain. The presence of pieces of different HV regions in a single insect indicates multiple Wolbachia infections. An NCR from OH has HV regions from all 4 primary strains. It remains to be determined if a 4X (or greater) multiple infection is the rule or an exception. 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