MOLECULAR IDENTIFICATION AND PHYLOGENETIC ANALYSES OF UNIQUE MICROORGANISMS FROM SWINE FECES AND MANURE STORAGE PITS
Location: Bioenergy Research Unit
Title: Robinsonia peoriae Gen. Nov., Sp. Nov., Isolated from a Swine-manure Storage Pit and a Human Clinical Source
Submitted to: International Journal of Systematic and Evolutionary Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: May 17, 2008
Publication Date: January 1, 2009
Citation: Cotta, M.A., Whitehead, T.R., Falsen, E., Moore, E., Lawson, P.A. 2009. Robinsoniella peoriensis Gen. Nov., Sp. Nov., Isolated from a Swine-manure Storage Pit and a Human Clinical Source. International Journal of Systematic and Evolutionary Microbiology. 59(1):150-155.
Interpretive Summary: Odor associated with swine farms is a nuisance and increasingly the target of regulation. Elucidation of the microbial processes that are responsible for the generation of odorous chemical emissions could lead to the development of intervention strategies. We have characterized the microbial populations of stored swine manure and discovered a number of previously unidentified bacteria. In the current study, a new organism is described. This bacterium was named Robinsonia peoriae after I. M. Robinson, a former ARS scientist who contributed much to the study of swine microbiology, and Peoria, the city where the organism was first isolated. Identifying the microorganisms present in stored manure and their role in production of specific odors is one way to gain a better understanding of this process and will help scientists working to solve this problem.
A polyphasic taxonomic study was performed on six strains of an unknown Gram-positive non-motile, spore-forming, short oval to rod-shaped bacterium isolated from a swine-manure storage pit. In addition to these strains, an isolate deposited in the Culture Collection, University of Goteborg (CCUG), was found to be biochemically related to the manure strains. The major end products of metabolism included acetate and succinate but not butyrate. Comparative 16S rRNA gene sequencing confirmed that all these isolates were highly related to each other and formed a hitherto unknown linage within the clostridial rRNA XIVa cluster of organisms. Based on phylogenetic and phenotypic evidence, it is proposed that the unknown bacterium be classified as a new genus and species, Robinsonia peoriae. The type strain is PC31**T (=CCUG 48729**T=NRRL B-23985**T).