ARS | Publication request: Genotyping American Bittersweet in Missouri with TRAP technique

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: October 30, 2007
Publication Date: January 12, 2008
Citation: Johnson, H.-Y., Li, R., Hu, J. 2008. Genotyping American Bittersweet in Missouri with TRAP Technique [abstract]. Plant & Animal Genome XVI Conference, January 12-16, 2008, San Diego, CA. P150.

Technical Abstract: American bittersweet (Celastrus Scandens) in the family of Celastraceae is a diecious woody and shrubby vine native to Missouri. As a result of aggressive invasion of a morphologically similar vine named Oriental Bittersweet (Celastrus orbiculatus) and subsequent hybridization, American bittersweet is becoming scarce and endangered for loosing its genetic identity. In an effort to identify and preserve true American Bittersweet, American Bittersweet plants were collected in Central Missouri for genotyping aided with Target Region Amplification Polymorphism (TRAP) technique. Plants examined in this study consist of known male and female plants and young plants of unknown gender grown from seeds. Five fixed primers designed from sequenced expressed sequence tags (EST) associated with high genetic conservation, flavanoid synthesis, and sex-linked genes were used for this study. Each primer was combined with four arbitrary primers in PCR reactions. The TRAP technique generated ample biomarkers for assessing genetic variability among plants tested. Results of this study ratify TRAP as a potentially useful technique for genotyping American Bittersweet.