INTERVENTIONS AND METHODOLOGIES TO REDUCE HUMAN FOOD-BORNE BACTERIAL PATHOGENS IN CHICKENS
Location: Poultry Microbiological Safety Research
Title: Efficacy of polymers in combination with biocides as sanitizer of salmonella inoculated broiler hatching eggs
Submitted to: Poultry International Exposition
Publication Type: Abstract Only
Publication Acceptance Date: November 15, 2007
Publication Date: July 20, 2008
Citation: Ritter, A.R., Buhr, R.J., Richardson, L.J., Cox Jr, N.A., Bright, W., Wilson, J.L. 2008. Efficacy of polymers in combination with biocides as sanitizer of salmonella inoculated broiler hatching eggs. Poultry International Exposition. 87(S1):151.
Salmonella contamination of broiler hatching eggs can be carried through the hatchery and with the hatched chick into the broiler house. Commercially available chemical hatching egg sanitizers have achieved acceptable levels of eggshell decontamination of >70% reductions when applied prior to setting. To evaluate the potential benefits of recently formulated combination chemicals (polymers in combination with multiple biocides) by Byotrol Inc. on eggshell decontamination a series of experiments was conducted. Replicate trials containing 10 eggs / chemical / trial were drip inoculated with a naladixic acid resistant Salmonella serovar Typhimurium (10^3 cfu/egg). After drying at room temperature for 1 h, eggs were spray sanitized with either water, hydrogen peroxide (common effective chemical), MC4 (2 quaternary ammoniums, a biquanide, and a bronopol associated in a polymer solution), NIP5 (4 quaternary ammoniums and a polyhexamethylenebiquanide hydrochloride associated in a polymer solution), OPF4 (4 quaternary ammoniums associated in a polymer solution), Polysphere (4 quaternary ammoniums associated in a polymer solution), or remained as an untreated positive control. In addition, the relative safety of these sanitizing chemicals (at the same concentrations) on embryo viability and hatchability was also evaluated. The incidence of Salmonella recovery was 86% for the untreated control eggs, 83% for water spray control, 9% for hydrogen peroxide, 20% for Polysphere, 7% for OPF4, and 0% for both MC4 and NIP5. Hatchability of fertile eggs sanitized with these chemicals was not significantly different (87 - 93%) when compared to the control value of 91%. Furthermore, hatched chick quality of eggs sprayed with these chemicals was also not different from that of the controls. Combination chemicals can effectively reduce eggshell Salmonella contamination and both MC4 and NIP5 consistently eliminated Salmonella present on eggshells below the level of recovery without any signs of a depression in hatchability or chick quality.