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United States Department of Agriculture

Agricultural Research Service

Research Project: PHYSIOLOGICAL APPROACHES TO INCREASE THE EFFICIENCY OF PORK PRODUCTION THROUGH IMPROVED NUTRITIONAL AND REPRODUCTIVE COMPETENCE

Location: Reproduction Research

Title: Placental transcriptome profile differences associated with selection for uterine capacity

Authors
item Freking, Bradley
item Miles, Jeremy
item Bischoff, Steven - NORTH CAROLINA STATE UNIV
item Xia, Yuannan - UNIVERSITY OF NEBRASKA
item Nonneman, Danny
item Vallet, Jeffrey
item Piedrahita, Jorge - NORTH CAROLINA STATE UNIV

Submitted to: Midwestern Section of the American Society of Animal Science
Publication Type: Abstract Only
Publication Acceptance Date: November 16, 2007
Publication Date: September 22, 2008
Repository URL: http://asas.org/abstracts/2008sectional/Supplement_3-38.pdf
Citation: Freking, B.A., Miles, J.R., Bischoff, S.R., Xia, Y., Nonneman, D.J., Vallet, J.L., Piedrahita, J.A. 2008. Placental transcriptome profile differences associated with selection for uterine capacity [abstract]. Journal of Animal Science. 86(E-Suppl. 3):49. Abstract #36.

Technical Abstract: Selection for 11 generations for uterine capacity (UC) resulted in 1.6 more live pigs born with no change in birth and placental weights. It was determined that the critical time period for the difference in litter size was established between d 25 and 45 of gestation. Our objective was to gain insight into placental transcriptional changes after selection for uterine capacity. Thirty gilts from the UC and control (CO) lines were subjected to unilateral hysterectomy-ovariectomy at approximately 160 d of age and mated within line at approximately 280 d. Gilts were slaughtered at d 25, 30, or 40 of gestation. Fetal and placental tissues were obtained from each live embryo. Two male and two female embryos closest to the litter mean for placental weight were chosen to represent each litter (n = 3 litters per line and time point combination). Placental tissues were pooled within litter and total RNA was extracted. Samples were labeled and hybridized to Affymetrix porcine array chips (n = 18) using manufacturers suggested protocols. Signal intensities were normalized using GC content Robust Multi-array Average (GCRMA) on the probe level data. Two-way ANOVA (two lines and three stages) was performed. Threshold values were set at a minimum of 1.5 fold difference and the false discovery rate was set to P < 0.05 (Benjamini and Hochberg algorithm). A total of 4171 targets on the array exceeded P value and threshold limits for the main effect of stage (2230 up-regulated and 1839 down-regulated from d 25 to d 40). Two targets, LIM domain and actin-binding protein 1 (LIMA1) and dedicator of cytokinesis 4 (DOCK4) approached significance for line (P < 0.08). Both genes appear to play roles in cell migration, suggesting participation in placental folded bilayer formation.

Last Modified: 8/1/2014