ARS | Publication request: Gene expression profiling of soybean near-isogenic lines contrasting in seed protein and oil

Submitted to: Plant and Animal Genome Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: November 26, 2007
Publication Date: January 12, 2008
Citation: Bolon, Y.E., Diers, B., Graham, M.A., Muehlbauer, G., Shoemaker, R.C., Specht, J., Vance, C.P. 2008. Gene expression profiling of soybean near-isogenic lines contrasting in seed protein and oil [abstract]. XVI Plant and Animal Genome Conference Proceedings, January 12-16, 2008, San Diego, California. Abstract W481.

Technical Abstract: Soybean profitability is affected by protein and oil content. Thus, an understanding of the genetic controls on protein and oil yield is important for future soybean improvement. In this study, we used Affymetrix soybean genome arrays with >37,500 Glycine max probe sets to compare gene expression profiles from nearly identical soybean lines that differ in protein and oil. Gene expression profiles were obtained from four different stages of the developing soybean seed. Analysis of differential gene expression produced a significant list of less than 200 genes. Presumably, these variations are related to the difference in protein and oil content between these two lines. These results demonstrate the power of gene expression analysis to contrast near-isogenic lines. Further investigation may provide new insight into the genes and pathways involved in protein and oil accumulation in the soybean seed.