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United States Department of Agriculture

Agricultural Research Service

Research Project: AUGMENTATIVE BIOLOGICAL CONTROL AND MASS REARING FOR BENEFICIAL AND PEST INSECTS

Location: Biological Control of Pests Research Unit

Title: PiggyBac Transgene Insertion Site GIZA Strain

Author
item Allen, Margaret

Submitted to: Genbank
Publication Type: Other
Publication Acceptance Date: October 20, 2007
Publication Date: December 1, 2008
Citation: Allen, M. L. 2008. PiggyBac Transgene Insertion Site GIZA Strain. Genbank. EU143852.

Technical Abstract: Genetically modified New World screwworms were analyzed by inverse PCR and several sequences of genomic DNA flanking the transposon insertion sites were obtained. One transgenic strain, designated GIZA, exhibited the properties of a lethal genetic mutation. Classic genetic crosses of these insects resulted in death of homozygous individuals. The 407 bases surrounding the transposon insert translated to an uninterrupted 132 amino acid sequence bearing similarity to other insect myosin genes.

Last Modified: 9/3/2014
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