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United States Department of Agriculture

Agricultural Research Service

Research Project: IMMUNOLOGIC AND PHARMACOLOGICAL INTERVENTIONS OF VECTOR-BORNE BABESIOSIS

Location: Animal Diseases Research

Title: Taking advantage of the polymorphism of the MSA-2 family for Babesia bovis strain characterization

Authors
item Wilkowsky, S - INTA-ARGENTINA
item Farber, M - INTA-ARGENTINA
item Echaide, I - INTA-ARGENTINA
item Mosqueda, J - INTA-ARGENTINA
item Alcaraz, E - INTA-ARGENTINA
item Suarez, Carlos
item Florin-Christensen, M - INTA-ARGENTINA

Submitted to: Parassitologia
Publication Type: Monograph
Publication Acceptance Date: April 15, 2007
Publication Date: May 1, 2007
Citation: Wilkowsky, S., Farber, M., Echaide, I., Mosqueda, J., Alcaraz, E., Suarez, C.E., Florin-Christensen, M. 2007. Taking advantage of the polymorphism of the MSA-2 family for Babesia bovis strain characterization. Parassitologia. 49(Suppl. 1):63-66.

Interpretive Summary: The Merozoite Surface Antigen-2 (MSA-2) family of Babesia bovis is a group of variable genes which share conserved sequence. In this work, we have analyzed the sequences of the msa-2a1, a2 and 2b genes in two geographically distant strains from Mexico and Argentina and detected a certain degree of genotypic diversity that can be exploited for the development of a new molecular tool for the discrimination of B. bovis field samples. Here, we describe a PCR restriction assay based on the msa2-a1, -a2 and -2b genes of B. bovis. When field strains from Argentina, Mexico and USA were analyzed, the results showed a strain-specific band pattern indicating the presence of differentially located BspMI restriction sites. This approach provides a simple method for the genotyping/strain differentiation of B. bovis field samples.

Technical Abstract: The Merozoite Surface Antigen-2 (MSA-2) family of Babesia bovis is a group of variable genes which share conserved 5' and 3' conserved ends. These genes encode membrane anchored glycoproteins, named MSA-2a1, a2, b and c, which are immunodominant antigens located on the surface of sporozoites and merozoites. In this work, we have analyzed the sequences of the msa-2a1, a2 and 2b genes in two geographically distant strains from Mexico and Argentina and detected a certain degree of genotypic diversity that can be exploited for the development of a new molecular tool for the discrimination of B. bovis field samples. Here, we describe a PCR restriction assay based on the msa2-a1, -a2 and -2b genes of B. bovis. When field strains from Argentina, Mexico and USA were analyzed, the results showed a strain-specific band pattern indicating the presence of differentially located BspMI restriction sites. This approach provides a simple method for the genotyping/strain differentiation of B. bovis field samples

Last Modified: 7/24/2014
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