Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: October 17, 2007
Publication Date: October 30, 2007
Citation: Liu, Z. 2007. Validation and standardization of gene expression data for microarray and real time quantitative PCR using universal external RNA controls [abstract]. Cambridge Healthtech Institute Conference on Quantitative PCR, Microarrays, and Biological Validation.
This presentation will introduce newly developed universal external ribonucleic acid (RNA) controls and their applications on different platforms of microarray and quantitative real time polymerase chain reaction (qRT-PCR) including SYBR Green® and TaqMan® probe-based chemistries. Data obtained from different platforms will be presented as an example to demonstrate uniqueness of the control and data comparability derived from microarray and qRT-PCR using the same source of RNA and the universal controls. Application of the universal controls has changed the conventional practice of quantitative PCR and made it possible as a high throughput assay platform for gene expression analysis. The universal control is the first of its kind and filled the gap of lacking a reliable, independent, and robust standard for quantitative gene expression analysis using different platforms.