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United States Department of Agriculture

Agricultural Research Service

Research Project: GENOMICS AND ENGINEERING OF STRESS-TOLERANT MICROBES FOR LOWER COST PRODUCTION OF BIOFUELS AND BIOPRODUCTS

Location: Crop Bioprotection Research

Title: Validation and standardization of gene expression data for microarray and real time quantitative PCR using universal external RNA controls

Author
item Liu, Zonglin

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: October 17, 2007
Publication Date: October 30, 2007
Citation: Liu, Z. 2007. Validation and standardization of gene expression data for microarray and real time quantitative PCR using universal external RNA controls [abstract]. Cambridge Healthtech Institute Conference on Quantitative PCR, Microarrays, and Biological Validation.

Technical Abstract: This presentation will introduce newly developed universal external ribonucleic acid (RNA) controls and their applications on different platforms of microarray and quantitative real time polymerase chain reaction (qRT-PCR) including SYBR Green® and TaqMan® probe-based chemistries. Data obtained from different platforms will be presented as an example to demonstrate uniqueness of the control and data comparability derived from microarray and qRT-PCR using the same source of RNA and the universal controls. Application of the universal controls has changed the conventional practice of quantitative PCR and made it possible as a high throughput assay platform for gene expression analysis. The universal control is the first of its kind and filled the gap of lacking a reliable, independent, and robust standard for quantitative gene expression analysis using different platforms.

Last Modified: 7/31/2014