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United States Department of Agriculture

Agricultural Research Service

Title: Whole genome mapping and QTL analysis in a doubled haploid population derived from the cross between a synthetic hexaploid wheat and hard red spring wheat

Authors
item Chu, Chenggen - NORTH DAKOTA STATE UNIV.
item Xu, Steven
item Friesen, Timothy
item Faris, Justin

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: October 5, 2007
Publication Date: December 1, 2007
Citation: Chu, C., Xu, S.S., Friesen, T.L., Faris, J.D. 2007. Whole genome mapping and QTL analysis in a doubled haploid population derived from the cross between a synthetic hexaploid wheat and hard red spring wheat. Meeting Abstract. National Wheat Conference Abstract pg 20.

Technical Abstract: Quantitative trait loci (QTL) analysis allows the identification of genomic regions associated with quantitative traits, which provides an estimation of the number and chromosomal location of genes involved and leads to the identification of molecular markers suitable for marker-assisted selection (MAS). In this research, we used the wheat × maize method to develop a doubled haploid (DH) population derived from the synthetic hexaploid wheat line TA4152-60 and the North Dakota hard red spring wheat line ND495. The population consisted of 213 lines, and a subset of 120 lines was randomly selected and used to construct linkage maps of all 21 chromosomes. The maps consisted of 626 markers including 408 SSRs and 218 TRAPs, and spanned 3,811.5 cM with an average density of one marker per 6.1 cM. Telomere sequence-based fixed TRAP primers were used to define the ends of seven linkage groups. Novel tan spot resistance QTLs were identified on chromosomes 2A, 5A, and 5B. In addition to Tsn1 and Snn1, a new Stagonospora nodorum blotch (SNB) toxin sensitivity gene identified on chromosome 3D was found to be significantly associated with the disease. Major QTL for days to heading, plant height, coleoptile color, glume toughness, and seed threshability were also identified. The DH population and genetic map will be a useful tool for the identification of other disease resistance QTL and agronomically important loci, as well as aid in the identification and development of markers for MAS.

Last Modified: 8/22/2014
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