Submitted to: Journal of Biomedicine and Biotechnology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: February 14, 2010
Publication Date: April 20, 2010
Citation: Klink, V.P., Overall, C.C., Alkharouf, N.W., Macdonald, M.H., Matthews, B.F. 2010. Microarray detection calls as a means to compare transcripts within syncytial cells isolated from incompatible and compatible soybean (Glycine max) roots infected by the soybean cyst nematode (Heterodera glycines). Journal of Biomedicine and Biotechnology. 2010(2010):Article ID 491217 30 pages.
Interpretive Summary: The soybean cyst nematode causes almost one billion dollars in losses to the soybean crop each year in the USA. Better understanding of the resistance and susceptible responses of soybean to nematodes may provide new approaches to developing nematode-resistant soybean. We used a combination of new techniques to identify genes expressed in specific cells upon which the nematode feeds. One technique, laser capture microdissection, was used to isolate cells from which soybean nematodes obtain their nourishment. The second technique, microarrays, was used to identify the presence or absence of specific gene transcripts (mRNA). Thus, we determined which genes are expressed in these cells. This information will be useful to scientists working to improve resistance of plants to nematodes using biotechnology and engineering.
Laser capture microdissection (LCM) was used to isolate pericycle and syncytial cells in soybean (Glycine max cultivar [cv.] Peking) roots infected by incompatible or compatible populations of soybean cyst nematode (SCN [Heterodera glycines]). Gene transcript detection calls were assayed using the Affymetrix® soybean GeneChip®. Our analyses identified the presence of 13,291 transcripts between the different sample types. These transcripts filtered down into a total of 6,267 that were detected as being present in one or more sample types. Many of these were found specifically in compatible or incompatible syncytial cell samples from three days post infection (dpi). These analyses also have identified additional transcripts found in three dpi or eight dpi compatible syncytial cells,
demonstrating further that gene expression is changing over the course of syncytial cell development as it matures into a functional feeding site.