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United States Department of Agriculture

Agricultural Research Service

Title: Co-dominant SCAR Markers for Detection of the Ty-3 and Ty-3a Loci from Solanum chilense at 25 cM of Chromosome 6 of Tomato

Authors
item Ji, Yuanfu - UN OF FL WIMAUMA
item Salus, Melinda - UN OF WI MADISON
item Van Betteray, Bram - NUNHEMS THE NETHERLANDS
item Smeets, Josie - NUNHEMS THE NETHERLANDS
item Jensen, Katie - UN OF WI MADISON
item Martin, Christopher - UN OF WI MADISON
item Mejia, Luis - UN DE SAN CARLOS GUATEMAL
item Scott, Jay - UN 0F FL WIMAUMA
item Havey, Michael
item Maxwell, Douglas - UN OF WI MADISON

Submitted to: Tomato Genetics Cooperative
Publication Type: Research Notes
Publication Acceptance Date: November 5, 2007
Publication Date: March 15, 2008
Citation: Ji, Y., Salus, M.S., Van Betteray, B., Smeets, J., Jensen, K.S., Martin, C.T., Mejia, L., Scott, J.W., Havey, M.J., Maxwell, D.P. 2008. Co-dominant SCAR Markers for Detection of the Ty-3 and Ty-3a Loci from Solanum chilense at 25 cM of Chromosome 6 of Tomato. Tomato Genetics Cooperative. 57:25-29.

Technical Abstract: Breeding for resistance to begomoviruses in tomato can be greatly aided by the availability of PCR-based markers for the various resistance loci. Four begomovirus-resistance loci or regions have been mapped to chromosome 6 (Agrama and Scott, 2006; Chagué et al., 1997; Ji and Scott, 2006b; Ji et al., 2007; Zamir et al., 1994). Ji and Scott reported the development of SCAR and CAPS markers linked to begomovirus resistance genes derived from S. chilense on chromosome 6, and they determined that the Ty-3 locus mapped to a region that included the FER locus. Jensen and Maxwell found that the sequences for the G8 gene of the BAC clone 56B23 are different for lines derived from LA2779 and LA1932. To differentiate the two introgressions, the one from LA2779 is designated Ty-3 and the one from LA1932, Ty-3a. This report describes two sets of PCR primers that provide co-dominant SCAR markers for detection of the Ty-3 and Ty-3a introgressions.

Last Modified: 4/19/2014
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