ARS | Publication request: Transcriptome analysis of the wheat-Puccinia striiformis f. sp. tritici interaction during both Yr5-mediated race-specific resistance and basal defense

Submitted to: American Phytopathological Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: May 1, 2007
Publication Date: July 1, 2007
Citation: Coram, T., Chen, X. 2007. Transcriptome analysis of the wheat-Puccinia striiformis f. sp. tritici interaction during both Yr5-mediated race-specific resistance and basal defense. American Phytopathological Society Abstracts, San Diego, CA, 7/28-8/2/07, 97:S24.

Technical Abstract: The Wheat GeneChip® was used to profile the changes occurring in two wheat isolines that differed for the presence of the Yr5 gene after inoculation with the stripe rust pathogen Puccinia striiformis Westend. f. sp. tritici Eriks. Yr5 represents a single R gene that confers all-stage resistance to all currently known North American races of P. s. tritici. This time-course study identified numerous defense-related transcripts that were important for R-gene-mediated race-specific resistance as well as basal defense, and supported a model that links P. s. tritici recognition to the expression of plant defense responses. The presence of Yr5 resulted in a rapid and amplified resistance response involving signaling pathways and defense-related transcripts known to occur during R¬-gene-mediated responses. Basal defense also involved substantial induction of many defense-related transcripts but the lack of R-gene signaling resulted in a delayed and weaker response, most likely due to the absence of increased cellular signaling and nitric oxide production that were able to confer an effective hypersensitive response in the presence of Yr5. The data generated provides novel insights into the cellular mechanisms of wheat defense to an economically important pathogen, and the findings will be useful for the development of durable resistant cultivars.