Technical Abstract: In the dry grind process, corn starch is converted into sugars which are fermented into ethanol. The remaining corn components (protein, fiber, fat, and ash) form coproduct, distillers dried grains with solubles (DDGS). The combination of sieving and elutriation (air classification), known as elusieve process, was found to be effective in separating fiber from DDGS. In this study, elusieve fiber was evaluated for ethanol production and results were compared with those reported in other studies for fiber from different corn processing techniques. Fiber samples were pretreated using acid hydrolysis followed by enzymatic treatment. The hydrolyzate was fermented using Escherichia coli FBR5 strain. Efficiency of ethanol production from elusieve fiber was 89 to 91%, similar to that for pericarp fiber from wet milling and quick fiber processes (86 to 90%). Ethanol yields from elusieve fiber were 0.23 to 0.25 L/kg (0.027 to 0.030 gal/lb); similar to ethanol yields from wet milling pericarp fiber and quick fiber. Fermentations were completed within 50 hr. Implementation of elusieve fiber conversion to ethanol within a dry grind plant may not be currently economically feasible due to the small scale of operation. Ethanol production from elusieve fiber in a 25 MMGY lignocellulose to ethanol plant would fetch 12 to 57% higher price for elusieve fiber than if it was used as animal feed. Elusieve fiber conversion could result in 1.2 to 2.7% increase in ethanol production from dry grind plants.