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United States Department of Agriculture

Agricultural Research Service

Title: Genetic diversity in conventional and synthetic wheats with drought and salinity tolerance based on AFLP

Authors
item Das, Modan
item BAI, GUIHUA
item Kazi, Mujeeb - CIMMYT

Submitted to: Canadian Journal of Plant Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: May 1, 2007
Publication Date: October 1, 2007
Citation: Das, M.K., Bai, G., Kazi, M. 2007. Genetic diversity in conventional and synthetic wheats with drought and salinity tolerance based on AFLP. Canadian Journal of Plant Science. 87:691-702.

Interpretive Summary: Genetic diversity is an important foundation for breeding cultivars with desired traits. A total of 14 drought and 27 salinity tolerant conventional and synthetic wheat accessions were assessed using amplified fragment length polymorphism (AFLP) markers. A total of 918 AFLP markers were evaluated and more than 300 markers were polymorphic across all 41 wheat accessions. Cluster and principal component analysis showed that both drought and salinity tolerant accessions have different genetic makeup within each group. These accessions possess a substantial amount of genetic diversity within each group and would be very valuable materials to be used in breeding wheat for drought and salinity tolerance.

Technical Abstract: Genetic diversity, among fourteen drought and twenty-seven salinity tolerant conventional and synthetic wheat (Triticum sp.) accessions containing different sources of the D genome, was assessed using amplified fragment length polymorphism (AFLP). The wheat accessions were analyzed with 20 EcoRI/MseI primer combinations. Among 918 fragments scored, 368 were polymorphic across all 41 wheat accessions, 348 were polymorphic among the accessions with drought tolerance and 310 were polymorphic among the accessions with salinity tolerance. Similarity coefficients among all accessions based on Jaccard’s coefficient ranged from 0.18 to 0.92 with an average of 0.53 ± 0.01; among drought tolerant accessions, from 0.16 to 0.79 with an average of 0.43 ± 0.02; and among salinity tolerant accessions, from 0.16 to 0.92 with an average of 0.57 ± 0.01. Polymorphic information content (PIC) among all accessions ranged from 0.05 to 0.50 with an average PIC of 0.30 ± 0.01; among drought tolerant accessions, from 0.13 to 0.50 with an average PIC of 0.37 ± 0.01; and among salinity tolerant accessions, from 0.07 to 0.50 with an average PIC of 0.29 ± 0.01. Cluster and principal component analysis showed distinct groups of accessions both within drought and salinity tolerant entries. These accessions possess a substantial amount of genetic diversity and would be very valuable materials for breeding wheat with drought and salinity tolerance.

Last Modified: 9/10/2014
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