Technical Abstract: The effect of a Lactobacillus-based probiotic (FM-B11**TM) on Salmonella recovery was evaluated in liquid (Exp. 1) and lyophilized (Exp. 2) forms in two separate experiments with two trials each. For each trial, eighty broiler chicks were randomly allocated into two treatments: control and probiotic culture. All chicks were challenged with S. enteritidis (SE) upon arrival at our laboratory. In both experiments, probiotic culture was administered in the drinking water for three consecutive days beginning one hour after SE challenge. Cecal tonsils were aseptically removed at 24 and 72 h post-challenge, followed by enrichment and plating on XLD agar for the presence or absence of Salmonella-typical colonies. In Exp. 1, a significant reduction (P<0.05) of SE positive samples was observed in both trials at 24 and 72 h post-challenge. Additionally, in Exp. 2, the lyophilized probiotic decreased (P<0.05) SE recovery at both 24 and 72 h post-challenge compared to the control group in trial 1. In trial 2, SE evaluation was performed only at 72 h after challenge and fewer (P<0.001) treated samples were SE positive. Results showed that application of either liquid or lyophilized probiotic culture for three consecutive days in the drinking water may help to reduce SE recovery from young birds, although further research is needed to elucidate the mechanism of this response.