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Title: New finding and optimal production of a novel extracellular alkaline lipase from Yarrowia lipolytica NRRL Y-2178

Author
item LEE, GEON-HO - KYUNGPOOK NATL UNIV KOREA
item BAE, JAE-HAN - KYUNGPOOK NATL UNIV KOREA
item SUH, MIN-JUNG - KYUNGPOOK NATL UNIV KOREA
item KIM, IN-HWAN - KOREA UNIV, SEOUL KOREA
item Hou, Ching
item KIM, HAK-RYUL - KYUNGPOOK NATL UNIV KOREA

Submitted to: Journal of Microbiology and Biotechnology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/6/2007
Publication Date: 7/20/2007
Citation: Lee, G., Bae, J., Suh, M., Kim, I., Hou, C.T., Kim, H. 2007. New finding and optimal production of a novel extracellular alkaline lipase from Yarrowia lipolytica NRRL Y-2178. Journal of Microbiology and Biotechnology. 17:1054-1057.

Interpretive Summary: Lipases from various sources have been widely used in industrial applications, such as in food, chemical, pharmaceutical and detergent industries. We screened 17 lipase-producing yeast strains, which were pre-screened for substrate specificity of lipase from more than 500 yeast strains from the Agricultural Research Service Culture Collection (Peoria, IL, USA), and selected Yarrowia lipolytica NRRL Y-2178 as the best lipase producer. This report presents new findings on optimal production of a novel extracellular alkaline lipase from Y. lipolytica NRRL Y-2178. Results obtained suggest that Y. lipolytica NRRL Y-2178 has good industrial potential as a new alkaline lipase producer.

Technical Abstract: Lipases are industrially and useful versatile enzymes that catalyze numerous different reactions including hydrolysis of triglycerides, transesterification, and chiral synthesis of esters under natural conditions. Although lipases from various sources have been widely used in industrial applications, such as in food, chemical, pharmaceutical and detergent industries, there are still substantial current interests in developing new microbial lipases, specifically those functioning in abnormal conditions. We screened 17 lipase-producing yeast strains, which were pre-screened for substrate specificity of lipase from more than 500 yeast strains from the Agricultural Research Service Culture Collection (Peoria, IL, USA), and selected Yarrowia lipolytica NRRL Y-2178 as the best lipase producer. This report presents new findings and optimal production of a novel extracellular alkaline lipase from Y. lipolytica NRRL Y-2178. Optimal culture conditions for lipase production by Y. lipolytica NRRL Y-2178 were 72 hours incubation time, 27.5', and pH 9.0. Glycerol and glucose were efficiently used as carbon sources and a combination of yeast extract and peptone was a good nitrogen source for lipase production by Y. lipolytica NRRL Y-2178. These results suggest that Y. lipolytica NRRL Y-2178 has industrial potential as a new alkaline lipase producer.