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United States Department of Agriculture

Agricultural Research Service

Title: Cytoprotective effects of anthocyanins and other phenolic fractions of Boysenberry and blackcurrant on dopamine and amyloid Beta induced oxidative stress in transfected COS-7 cells

Authors
item Ghosh, Dilip - HORT. & FOOD INSTI.OF NZ
item Mcghie, Tony - HORT. & FOOD INSTI. OF NZ
item Fisher, Derek
item Joseph, James

Submitted to: Journal of the Science of Food and Agriculture
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: March 1, 2007
Publication Date: August 30, 2007
Citation: Ghosh, D., Mcghie, T.K., Fisher, D.R., Joseph, J.A. 2007. Cytoprotective effects of anthocyanins and other phenolic fractions of Boysenberry and blackcurrant on dopamine and amyloid Beta induced oxidative stress in transfected COS-7 cells. Journal of the Science of Food and Agriculture. 87:2061-2067.

Interpretive Summary: There is a growing interest both from consumers and researchers in the role that berries play in human health. In the experiments reported here, we assessed the ability of Boysenberry and blackcurrant to ameliorate the deleterious effects of two different oxidative stressors (amyloid(Beta)25 – 35 and dopamine) given to cells. The number of cells that survived the different treatments was also studied. Out results demonstrate that extracts of Boysenberry and blackcurrant showed significant positive effect and counteracted the oxidative stressors in the cells. Blackcurrant showed slightly higher protective effect against dopamine, whereas Boysenberry had a higher effect against the amyloid(Beta)25 – 35. In the cell survival studies, both Boysenberry and blackcurrant showed significant protective effects with both oxidative stressors. Our results provide further evidence for the protective effects of berries against neurotoxins in brain cells.

Technical Abstract: There is growing interest both from consumers and researchers in the role that berries play in human health. In the experiments reported here, we assessed the ability of anthocyanins and phenolic fractions of Boysenberry and blackcurrant to ameliorate the deleterious effect of the amyloid(Beta)25 – 35 (100 umolL-1, 24h) and dopamine (1mmolL-1, 4 h) on calcium buffering (recovery) of M1 muscarinic receptor-transfected COS-7 cells. Cell viability was also studied. Our results demonstrate that extracts of Boysenberry and blackcurrant showed significant protective effect and restored the calcium buffering ability of cells that had been subjected to oxidative stress induced by dopamine and the amyloid (Beta)25 – 35. Blackcurrant polyphenolics showed slightly higher protective effect against dopamine, whereas Boysenberry polyphenolics had a higher effect against the amyloid (Beta)25 – 35. In viability studies, all extracts showed significant protective effects against dopamine and amyloid(Beta)25 – 35-induced cytotoxicity. Our results provide further evidence for the protective effects of berries against the neurotoxic effect of dopamine and amyloid(Beta)25 – 35 in brain cells.

Last Modified: 10/1/2014
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