|Riga, Ekaterini - WA ST UNIV|
Submitted to: Journal of Nematology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: September 1, 2007
Publication Date: December 1, 2007
Citation: Mojtahedi, H., Boydston, R.A., Crosslin, J., Brown, C.R., Riga, E., Anderson, T.L., Spellman, D.E., Quick, R.A. 2007. Establishing a Corky Ringspot Disease Plot for Research Purposes. Journal of Nematology. 39(4):313-316. Interpretive Summary: A method to establish two experimental corky ringspot disease (CRS) plots that had no prior CRS history is described
Technical Abstract: A method to establish two experimental corky ringspot disease (CRS) plots that had no prior CRS history is described. CRS is a serious disease of potato in the Pacific Northwest USA, caused by tobacco rattle virus (TRV) and transmitted primarily by Paratrichodorus allius. ‘Samsun NN’ tobacco seedlings were inoculated with viruliferous P. allius in the greenhouse before they were transplanted into the field soil at the rate of 3,000 plus seedlings/ha. Care was taken to keep soil around plants in the greenhouse and transplants in the field moist to avoid vector mortality. The vector population in the soil was monitored by extraction, examination under microscope and bioassay on tobacco seedlings to ascertain that they were virus carriers. Presence of virus in tobacco bioassay plants was determined by visual symptoms on tobacco leaves and by testing leaves and roots using ELISA. After two years of planting infected tobacco seedlings, 100% of soil samples collected from these fields contained viruliferous P. allius. More importantly, all five commercial potato cultivars, known to be susceptible, were inflicted with CRS disease when grown in one of the test plots. In this plot, 82% of ‘Ranger Russet’ potato tubers were symptomatic with CRS, indicating that the operation was successful.