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United States Department of Agriculture

Agricultural Research Service

Title: Development of molecular markers for introgression of potato leafroll virus resistance from S. etuberosum into cultivated potato

Authors
item Kelley, Kevin
item Gillen, Anne
item Whitworth, Jonathan
item Novy, Richard

Submitted to: American Journal of Potato Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: July 25, 2006
Publication Date: January 1, 2007
Repository URL: http://riley.nal.usda.gov/nal_web/digi/submission.html
Citation: Kelley, K., Gillen, A.M., Whitworth, J.L., Novy, R.G. Development of molecular markers for introgression of potato leafroll virus resistance from S. etuberosum into cultivated potato. American Journal of Potato Research. 84(1):97

Technical Abstract: Potato leafroll virus (PLRV) is a major virus pathogen of potato. Infection of potato can result in substantial reductions in yield as well as tuber quality via the expression of net necrosis. Solanum etuberosum, a wild relative of cultivated potato, is highly resistant to PLRV as well as other potato diseases and insect pests. Reproductively isolated from cultivated potato, S. etuberosum, was somatically hybridized with a S. tuberosum haploid x S. berthaultii hybrid. Successive backcrossing of the somatic hybrid to cultivated potato has produced progeny with improved agronomics and PLRV resistance from S. etuberosum. Segregation of PLRV resistance in a BC3 population suggests a monogenic trait. Using RFLP and CAPS markers, previously mapped in potato and tomato, PLRV resistance was localized to chromosome 4 in the segregating BC3 population. Having localized the genomic region associated with PLRV resistance, we report on our efforts in the identification of PCR-based molecular markers that may be used for marker-assisted selection for PLRV resistance.

Last Modified: 10/1/2014