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United States Department of Agriculture

Agricultural Research Service

Title: Bean pod mottle virus movement in insect feeding resistant soybeans

Authors
item Redinbaugh, Margaret
item Todd, Jane
item Hoy, C - OSU
item Hogenhout, S - OSU

Submitted to: American Society for Virology Meeting
Publication Type: Abstract Only
Publication Acceptance Date: January 31, 2007
Publication Date: July 1, 2007
Citation: Redinbaugh, M.G., Todd, J.C., Hoy, C.W., Hogenhout, S.A. 2007. Bean pod mottle virus movement in insect feeding resistant soybeans. American Society for Virology Meeting. p.22-27.

Technical Abstract: Maize fine streak virus (MFSV) is a plant-infecting nucleorhabdovirus that is transmitted by the black-faced leafhopper (Graminella nigrifrons [Forbes] Hemiptera: Cicadellidae). Plant-infecting rhabdoviruses are transmitted in a persistent propagative manner, meaning that the vector is also a host of the virus. The leafhopper transmitted MFSV to maize, teosinte, barley, oats wheat, rye and several cool season grasses, but not to Sorghum species. In these experiments, the plant host range of MFSV reflected the plant host range of its insect vector. In initial experiments, the rate of MFSV transmission by adult G. nigrifrons adults to maize was less than 2%. However, about 17% of the insects were infected with MFSV, as indicted by enzyme-linked immunosorbant assay (ELISA) of single insects. These results indicate that G. nigrifrons is an inefficient vector of MFSV. Transmission by nymphs was more efficient than by adults, and increasing the acquisition access and latent periods increased the incidence of infection and transmission for both nymphs and adults. No difference in transmission rate was noted for male and female leafhopper nymphs or adults. MFSV titer in leafhoppers that acquired and transmitted virus to maize was higher than in leafhoppers that were infected with MFSV, but did not transmit it. Since not all leafhoppers become infected with the virus and not all infected leafhoppers are vectors, we hypothesize that there are multiple barriers to MFSV replication and/or movement in G. nigrifrons.

Last Modified: 9/22/2014
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