Submitted to: Immunological Investigations
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: August 24, 2007
Publication Date: September 1, 2007
Citation: Waters, W.R., Sacco, R.E. 2007. Veterinary Applications for Monitoring Mononuclear Cell Proliferation Using Cell Tracking Dyes. Immunological Investigations. 36(5-6):887-908. Interpretive Summary: Understanding host responses to infection and/or vaccination by livestock and relevant wildlife species is critical for identifying strategies to control disease. This article describes new technologies for understanding how an animal responds to infection and/or vaccination. It describes basic aspects of the host immune response. Findings presented in this study should provide insight into new strategies to prevent disease through an improved understanding of how a cow, pig, white-tailed deer, or elk responds to infection/vaccination.
Technical Abstract: Veterinary species offer unique opportunities for the study of immune responses during natural host/pathogen interactions. In particular, experimental studies can be used to characterize the response to infection, vaccination, and influence of vaccination on the response to infection. The intent of this review is to demonstrate the use of dye tracking to monitor and characterize in vitro proliferative responses by mononuclear cell subsets from veterinary species as a surrogate to the in vivo response. Select findings demonstrate the usefulness of this approach to characterize unique cell types including various tissue dendritic cell populations,CD8aa+ T cells, gd T cells, and CD172a+ cells. Comparative approaches provide unique and comprehensive insights into mononuclear cell biology that may be applicable to similarly described cell populations in humans.