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United States Department of Agriculture

Agricultural Research Service

Research Project: EVALUATION OF COMPOUNDS AND STRATEGIES FOR CONTROLLING AQUATIC ANIMAL DISEASE

Location: Harry K. Dupree Stuttgart National Aquaculture Research Center

Title: Minimum inhibitory concentration testing of flavobacterium columnare

Authors
item Darwish, Ahmed
item Farmer, Bradley
item Hawke, John - LSU

Submitted to: Flavobacterium Meeting
Publication Type: Abstract Only
Publication Acceptance Date: April 5, 2007
Publication Date: May 2, 2007
Citation: Darwish, A.M., Farmer, B.D., Hawke, J.P. 2007. Minimum inhibitory concentration testing of flavobacterium columnare [abstract]. Flavobacterium 2007 Workshop Book of Abstracts. p.35.

Technical Abstract: A simple, accurate and reliable microdilution method has been developed to test the susceptibility of Flavobacterium columnare to antibiotics. The method has been used to determine the minimum inhibitory concentration (MIC) of 23 F. columnare isolates. The developed method conducted at 28 °C for 48 h used standardized inoculum, Mueller Hinton broth at 1/5 the full strength (4g/l), reference isolate, a positive and a negative control wells and a standardized custom made microtitre plates, Sensititre® Susceptibility Plates for Aquaculture (Trek Diagnostic Systems, Inc.). Escherichia coli, ATCC25922 was used as the reference isolate and produced MIC values within the range published by the Clinical and Laboratory Standards Institute (CLSI), formally the National Committee for Clinical Laboratory Standards (NCCLS). Mueller Hinton broth at 1/5 the full strength (4 g/l) was found to support significantly better growth for Escherichia coli, ATCC25922 and F. columnare type strain, ATCC23463, than Mueller Hinton broth at 1/7 the full strength.

Last Modified: 8/31/2014
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