Title: Immediate early responses of avian tracheal epithelial cells to infection with highly pathogenic avian influenza Authors
Submitted to: Meeting Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: June 15, 2007
Publication Date: October 23, 2007
Citation: Afonso, C.L., Sarmento, L., Pantin Jackwood, M.J., Kapczynski, D.R., Swayne, D.E. 2007. Immediate early responses of avian tracheal epithelial cells to infection with highly pathogenic avian influenza [abstract]. In: Proceedings of the International Symposium on Animal Genomics for Animal Health, October 23-25, 2007, Paris, France. p. 16. Technical Abstract: Highly pathogenic (HP) avian influenza viruses (AIV) present an on going threat to the U.S. poultry industry. In order to develop new AIV control strategies it is necessary to understand the underlying mechanism of viral infection. Because the early events of AIV infection can occur on tracheal epithelial cells (TEC) surfaces, we have developed chicken and duck tracheal epithelial cell systems to study immediate early host responses following AIV infection. Here we present the characterization of these systems and their use to study early responses to avian infection. TEC’s were highly susceptible to infection with AIV and other respiratory viruses. A 44K genes chicken microarray system was used to characterize the pattern of gene expression between control and infected TEC’s and to identify highly expressed molecular markers specific for avian tracheal epithelial cells. Growth curves in chicken and duck TEC’s were conducted for HP H5N1 Ck/Hong Kong/220/97 and Egret/Hong Kong/757.2/02 viruses that are highly lethal to chickens but differentially affect duck survival. Results indicate differential growth characteristics in vitro in duck and chicken TEC’s and distinct patterns of gene expression.