Technical Abstract: Conventional breeding techniques have increased the immediately-available sugar content in watermelon, up to 14%. This results in a sweet flavor but greatly limits the watermelon serving size for people concerned about dietary sugar intake. The Agricultural Research Service of the United States Department of Agriculture announces the release of MSW-28, a watermelon [Citrullus lanatus var. lanatus (Thunb.) Matsum. & Nakai)] line that exhibits medium sugar content (brix) and full flavor of heirloom varieties combined with the lycopene content and crisp texture of modern cultivars. MSW-28 is a midseason monoecious plant with 5 kg elongated fruit, with a medium to dark green rind and dark green stripes similar to 'Allsweet' and a thick tough rind, comparable to 'Black Diamond'. It has firm red flesh, an average brix content of 9.0, and an average lycopene content of 66 ug/g for ripe fruit, similar to 'Dixielee' in our fields. Taste tests showed that most of the taste testers preferred the sweeter fruit but there were a subset that consistently preferred the medium brix samples or ranked them the same as the control fruit. MSW-28 is a niche market fruit that will cater to consumers interested in moderate carbohydrate diets full of vitamins and phytonutrients.