DEVELOPMENT AND EVALUATION OF IMPROVED MATERIALS FOR MYCOTOXIN ANALYSIS
Location: Bacterial Foodborne Pathogens & Mycology Research Unit
Title: Recent developments in trichothecene analysis
| Pascale, Michelangelo - INST. SCIENCE & FOOD |
| Lippolis, Vincenzo - INST. SCIENCE & FOOD |
| Visconti, Angelo - INST. SCIENCE & FOOD |
Submitted to: American Chemical Society Symposium Series
Publication Type: Book / Chapter
Publication Acceptance Date: April 27, 2007
Publication Date: October 1, 2008
Citation: Pascale, M., Lippolis, V., Maragos, C.M., Visconti, A. 2008. Recent Developments in Trichothecene Analysis. American Chemical Society Symposium Series. 1001:192-210.
Interpretive Summary: Trichothecenes are a group of toxic metabolites and are produced by certain species of Fusarium that occur in cereal grains. This article describes recent advances at the Institute of Sciences of Food Production (ISPA, Bari, Italy) and the USDA-Agricultural Research Service (Peoria, IL, United States) in the development of techniques for measuring trichothecenes in foods. In particular, this manuscript reviews the literature on the use of liquid chromatographic (HPLC) methods and fluorescence polarization immunoassays for detecting trichothecenes such as deoxynivalenol (DON) and T-2 toxin in foods.
Trichothecenes are a group of toxic metabolites mainly produced by several Fusarium species occurring in cereals. Gas-chromatographic methods (GC/ECD or GC/MS) are widely used for quantitative determination of the more toxic type-A trichothecenes, i.e. T-2 and HT-2 toxins. Deoxynivalenol (DON), the main type-B trichothecene frequently occurring in wheat, is commonly detected by HPLC/UV with good accuracy and precision. Recently at ISPA-CNR, a new method has been developed for the simultaneous determination of T-2 and HT-2 toxins in cereal grains at ppb levels using immunoaffinity column clean-up, labeling with 1-anthroylnitrile, and HPLC with fluorescence detection (FD). Moreover, a fluorescence polarization (FP) immunoassay has been developed at USDA-ARS-NCAUR for rapid quantification of deoxynivalenol (DON) in wheat. Results of recent research on the improvement of methods for the determination of T-2 and HT-2 toxins in cereals by HPLC/FD, new labeling reagents (i.e. 1-naphthoyl chloride, 2-naphthoyl chloride, pyrene-1-carbonyl cyanide), and detection of T-2 by capillary electrophoresis/laser-induced fluorescence (CE-LIF) are presented, together with the optimization of the FP immunoassay for rapid screening of DON in common wheat, durum wheat, semolina, and pasta.