Technical Abstract: Trichothecenes are a group of toxic metabolites mainly produced by several Fusarium species occurring in cereals. Gas-chromatographic methods (GC/ECD or GC/MS) are widely used for quantitative determination of the more toxic type-A trichothecenes, i.e. T-2 and HT-2 toxins. Deoxynivalenol (DON), the main type-B trichothecene frequently occurring in wheat, is commonly detected by HPLC/UV with good accuracy and precision. Recently at ISPA-CNR, a new method has been developed for the simultaneous determination of T-2 and HT-2 toxins in cereal grains at ppb levels using immunoaffinity column clean-up, labeling with 1-anthroylnitrile, and HPLC with fluorescence detection (FD). Moreover, a fluorescence polarization (FP) immunoassay has been developed at USDA-ARS-NCAUR for rapid quantification of deoxynivalenol (DON) in wheat. Results of recent research on the improvement of methods for the determination of T-2 and HT-2 toxins in cereals by HPLC/FD, new labeling reagents (i.e. 1-naphthoyl chloride, 2-naphthoyl chloride, pyrene-1-carbonyl cyanide), and detection of T-2 by capillary electrophoresis/laser-induced fluorescence (CE-LIF) are presented, together with the optimization of the FP immunoassay for rapid screening of DON in common wheat, durum wheat, semolina, and pasta.