ARS | Publication request: Polymorphism among EST-based markers in tomato

Submitted to: Tomato Breeders Roundtable Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: April 13, 2006
Publication Date: N/A

Technical Abstract: Cultivated tomato (Lycopersicon esculentum Mill.) has a narrow genetic base. This is in part due to population genetic processes such as founder events, genetic bottlenecks, and natural and artificial selection during domestication. We characterize the nucleotide polymorphism in 26 EST-based markers, including Single Nucleotide Polymorphisms (SNPs) and Insertions/Deletions (Indels). Five of these markers are hypothesized to be cryptic wild species alleles (introgressions) within L. esculentum. These gene fragments were also resequenced in a diverse panel of 30 Plant Genetic Resources Unit (PGRU) tomato accessions, line TA496, and Lycopersicon peruvianum accession G 32591. The majority of sampled tomato accessions represented the primary center of diversity (Peru, Chile, and Ecuador), and countries contiguous with the primary center. Original collections were made between 1932 and 1976. These data will enhance our understanding of EST-based markers and the nature of genetic variation within cultivated tomato.