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United States Department of Agriculture

Agricultural Research Service

Research Project: EPIDEMIOLOGY, ECOLOGY, AND MOLECULAR GENETICS OF ANTIMICROBIAL RESISTANCE IN PATHOGENIC AND COMMENSAL BACTERIA FROM FOOD ANIMALS Title: Overview of USDA VetNet Campylobacter Database

Authors
item Jackson, Charlene
item Cray, Paula
item Douris, Aphrodite
item Pearson-Gresham, Cheryl
item Hall, Mary
item McGlinchey, Beth
item Plumblee, Jodie
item Price, Maria

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: April 13, 2007
Publication Date: April 16, 2007
Citation: Jackson, C.R., Cray, P.J., Douris, A., Pearson Gresham, C., Hall, M.C., Mcglinchey, B., Plumblee, J., Price, M.V. 2007. Overview of USDA VetNet Campylobacter Database. PulseNet Update Meeting. April 16 - 19, 2007. Providence, RI. Poster 26.

Technical Abstract: USDA VetNet commenced in March 2004 with submission of Salmonella isolates and in December 2005, Campylobacter isolates were added to the program. The objectives of USDA VetNet are to determine PFGE patterns of Salmonella and Campylobacter isolates submitted to the National Antimicrobial Resistance Monitoring System (NARMS), to compare USDA VetNet and PulseNet PFGE patterns, and to use the comparative data for surveillance and investigation of food-borne illness outbreaks. In addition, these data will also assist at the farm level through study of clonal dissemination. All isolates for the Campylobacter database originate from broiler slaughter plants. Isolates were restricted with a single enzyme, SmaI, and data were analyzed using Bionumerics. The Campylobacter database currently has 635 isolates resulting in 381 unique PFGE patterns. Campylobacter coli account for 187 (29%) of the isolates and 126 (33%) of the unique PFGE patterns. The majority of the isolates, 448 (71%), are C. jejuni which represent 255 (67%) of the unique PFGE patterns. The most common pattern for C. coli (9 isolates, 5%) is DBBS16.0003 while the most common pattern for C. jejuni (13 isolates, 3%) is DBRS16.0002. Future objectives for VetNet include web based access to enable participants to view and submit patterns.

Last Modified: 12/18/2014