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United States Department of Agriculture

Agricultural Research Service

Research Project: APPLICATION OF BIOLOGICAL AND MOLECULAR TECHNIQUES TO THE DIAGNOSIS AND CONTROL OF AVIAN INFLUENZA AND OTHER EMERGING POULTRY PATHOGENS

Location: Exotic and Emerging Avian Viral Diseases Research Unit

Title: Avian influenza neuraminidase 1 (N1) ELISA using baculovirus expressed antigen and its application on DIVA vaccination strategy

Authors
item Garcia, Maricarmen - UNIV GA, PDRC
item Liu, Yuru - UNIV GA, PDRC
item Swayne, David
item Suarez, David
item Jackwood, Mark - UNIV GA, PDRC
item Mundt, Egbert - UNIV GA, PDRC

Submitted to: American Veterinary Medical Association Abstract
Publication Type: Abstract Only
Publication Acceptance Date: May 1, 2007
Publication Date: July 14, 2007
Citation: Garcia, M., Liu, Y., Swayne, D.E., Suarez, D.L., Jackwood, M.W., Mundt, E. 2007. Avian influenza neuraminidase 1 (N1) ELISA using baculovirus expressed antigen and its application on DIVA vaccination strategy [abstract]. In: Convention Notes for the 144th Annual Convention of the American Veterinary Medical Association, July 14-19, 2007, Washington, DC. CDROM.

Technical Abstract: An ELISA was developed using baculovirus express N1 protein from the A/Chicken/Indonesia/11/03 (H5N1) virus. The objective of this study was to evaluate the specificity and sensitivity of the N1-ELISA. The specificity of the ELISA was tested with a chicken anti-sera panel raised against N1 to N9 virus subtypes. To evaluate the reactivity range of the N1-ELISA sera samples raised against N1 virus from the North America lineage were tested. The N1-ELISA was specific for the detection of N1 antibodies. The N1-ELISA was capable to detect N1 antibodies in chicken serum samples raised against North America H1N1 viruses (CK/NY/94, TK/SD/80, TK/NC/88). Antibodies against other neuraminidase subtypes (N2 to N9) did not cross-reacted with the N1 antigen. To evaluate the sensitivity of the N1-ELISA to detect N1 antibodies in vaccinated/infected chickens, serum samples collected from chickens vaccinated with H5N9, H5N2, and challenge with Asian H5N1 viruses, and samples from chickens vaccinated with H7 Pox vaccine and challenge with H7N1 will be tested by N1-ELISA. The N1-ELISA responses will be compared to hemagglutination inhibition (HI) titers and agar-gel precipitation (AGP).

Last Modified: 9/21/2014