ARS | Publication request: THE ISOAMYL OXIDASE GENE IN PENICILLIUM GRISEOFULVUM IS PART OF THE PATULIN BIOSYNTHETIC PATHWAY

Submitted to: Gordon Research Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: June 22, 2007
Publication Date: June 22, 2007
Citation: Dombrink Kurtzman, M. 2007. The isoamyl oxidase gene in penicillium griseofulvum is part of the patulin biosynthetic pathway [abstract]. Gordon Research Conference Proceedings.

Technical Abstract: Genes for the patulin biosynthetic pathway are likely to be arranged in a cluster, as is the case for other mycotoxins. GeneWalking was performed to identify genes both upstream and downstream of the isoepoxydon dehydrogenase (idh) gene in Penicillium griseofulvum NRRL 2159A. A gene with high sequence homology to the isoamyl alcohol oxidase (iao) gene was detected downstream of the idh gene. The newly identified gene coded for a secreted protein having a 22 amino acid signal peptide, a FAD-binding domain, and 11 potential N-linked glycosylation sites. Searches in GenBank indicated that Aspergillus terreus, A. oryzae, A. fumigatus and Gibberella zeae also contained genes coding for a putative isoamyl alcohol oxidase. This is the first report of an isoamyl alcohol oxidase gene in Penicillium.