Submitted to: Archives of Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: April 2, 2008
Publication Date: May 29, 2008
Citation: Vaira, A.M., Maroon-Lango, C.J., and Hammond, J. 2008. Molecular characterization of Lolium latent virus, proposed type member of a new genus in the family Flexiviridae. Archives of Virology. 153:1263-1270. Technical Abstract: Lolium latent virus (LoLV) was recently detected in the United States for the first time (Maroon-Lango et al., 2006, Plant Disease 90:528) in ryegrass hybrids (Lolium perenne x multiflorum). The genome of one U.S. isolate, LoLV-US1, has now been fully sequenced. The positive strand genomic RNA is 7674 nt long and is organized in five open reading frames encoding the replication-associated protein, the movement-associated triple gene block proteins and the capsid protein. This genome organization is similar to that of viruses in the genera Potexvirus and Foveavirus; however, phylogenetic analysis of the deduced amino acid sequences of the polymerase and the capsid proteins, together with the analysis of the complete LoLV genomic sequence highlights features peculiar to LoLV. These characteristics indicate that LoLV forms a monotypic group separate from existing genera-assigned and other unassigned species within the family Flexiviridae, for which we propose the genus name Lolavirus.