APPLICATION OF BIOLOGICAL AND MOLECULAR TECHNIQUES TO THE DIAGNOSIS AND CONTROL OF AVIAN INFLUENZA AND OTHER EMERGING POULTRY PATHOGENS
Location: Exotic and Emerging Avian Viral Diseases Research Unit
Title: Develop of new candidate H5N1 vaccine virus for pre-pandemic vaccine
| Dong, Jie - CHINA CDC, BEIJING |
| Matsuoka, Yumiko - CDC, ATLANTA, GA |
| Yu, Hongjie - CHINA CDC, BEIJING |
| Shu, Yuelong - CHINA CDC, BEIJING |
| Donis, Ruben - CDC, ATLANTA, GA |
| Cox, Nancy - CDC, ATLANTA, GA |
Submitted to: Options for the Control of Influenza Conference
Publication Type: Abstract Only
Publication Acceptance Date: April 1, 2007
Publication Date: June 17, 2007
Citation: Dong, J., Matsuoka, Y., Swayne, D.E., Yu, H., Shu, Y., Donis, R., Cox, N. 2007. Develop of new candidate H5N1 vaccine virus for pre-pandemic vaccine [abstract]. In: Abstracts of the Conference on the Options for the Control of Influenza VI, June 17-23, 2007, Toronto, Canada. p. 137.
Abstract This study describes the development of new candidate H5N1 vaccine by reverse genetics. Anhui/01/2005(H5N1)-PR8-IBCDC-RG5 (Anhui/PR8) virus was produced under the condition in compliance with Good Laboratory Practice (GLP). The virus contains 6 internal genes from A/Puerto Rico/8/34 (PR8) and the NA and modified HA genes from A/Anhui/01/2005(H5N1). The reassortant virus was rescued by transfection of plasmid DNAs into certified Vero cells. The HA and NA genes of rescued virus are identical to those of the parental virus except for the modified HA cleavage site where 4 basic amino acid residues were removed. The reassortant virus was trypsin-dependent for the formation of plaques in chicken embryo fibroblast (CEF) cells. The virus was also tested in mammalian (ferrets) and avian (chickens) hosts and was confirmed to be non-pathogenic. Thus Anhui/PR8 is suitable as a pre-pandemic vaccine candidate for the subsequent human safety study.