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United States Department of Agriculture

Agricultural Research Service

Title: A New Electrophoresis Technique to Seperate Microsatellite Alleles

Authors
item Wang, Xingwang - UNIV OF TENN
item Rinehart, Timothy
item Wadhl, Phillip - UNIV OF TENN
item Spiers, James
item Johnson, Denita - UNIV OF TENN
item Trigiano, Robert - UNIV OF TENN

Submitted to: African Journal of Biotechnology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: October 20, 2008
Publication Date: June 3, 2009
Citation: Wang, X., Rinehart, T.A., Wadhl, P.A., Spiers, J.M., Johnson, D., Trigiano, R.N. 2009. A New Electrophoresis Technique to Seperate Microsatellite Alleles Vol.8 (11), pp.2432-2436. African Journal of Biotechnology.

Interpretive Summary: Traditional agarose and polyacrylamide gel electrophoresis have been used commonly for microsatellite (simple sequence repeats, SSRs) analysis, but they are labor- intensive and not always able to provide accurate sizes for different alleles. Capillary sequencers provide automated analysis and accurate allele sizes; however, the costs of the instrument, reagents and labeled primers make the use of sequencers uneconomical for most routine microsatellite analysis. Here, we report a fast, cost-effective and accurate method for doing routine microsatellite array analysis using a compact, bench-top multi-capillary electrophoresis system, the HDA-GT12 TM Genetic Analyzer (eGene, Irvine, CA, USA). SSRs from flowering dogwood (Cornus florida L.) were used to compare the different types of electrophoresis.

Technical Abstract: Traditional agarose and polyacrylamide gel electrophoresis have been used commonly for microsatellite (simple sequence repeats, SSRs) analysis, but they are labor- intensive and not always able to provide accurate sizes for different alleles. Capillary sequencers provide automated analysis and accurate allele sizes; however, the costs of the instrument, reagents and labeled primers make the use of sequencers uneconomical for most routine microsatellite analysis. Here, we report a fast, cost-effective and accurate method for doing routine microsatellite array analysis using a compact, bench-top multi-capillary electrophoresis system, the HDA-GT12 TM Genetic Analyzer (eGene, Irvine, CA, USA). SSRs from flowering dogwood (Cornus florida L.) were used to compare the different types of electrophoresis.

Last Modified: 7/25/2014
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