|Oliver, C - NORTH DAKOTA STATE UNIV|
|Bauer, M - NORTH DAKOTA STATE UNIV|
|Caton, J - NORTH DAKOTA STATE UNIV|
Submitted to: American Chemical Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: December 16, 2006
Publication Date: March 25, 2007
Citation: Oliver, C.E., Bauer, M.L., Caton, J.S., Anderson, R.C., Smith, D.J. 2007. The in vitro reduction of sodium [36Cl]-chlorate in bovine ruminal fluid. American Chemical Society Spring Meetings, March 25-29, 2007, Chicago, IL Technical Abstract: Sodium chlorate effectively reduces or eliminates the numbers of gram-negative pathogenic bacteria in the gastrointestinal tracts of live cattle. Limitations to the in vivo efficacy of chlorate are its rapid absorption from the gastrointestinal tract and its presumed reduction to chloride within the gastrointestinal tract. We hypothesized that chlorate would be reduced via ruminal bacteria in a ruminal in vitro system, and that the reduction of chlorate would be influenced by the dietary forage:concentrate ratio, thus, four ruminally-cannulated steers were fed 20 or 80% concentrate diets in a cross-over design. Ruminal fluid was collected in two periods and dispensed into in vitro tubes containing sodium [36Cl]chlorate sufficient for 100 or 300 ppm final chlorate concentrations. Tubes were incubated for 0, 1, 4, 8, 16, or 24 h; autoclaved control ruminal fluid, fortified with sodium [36Cl]chlorate, was incubated for 24 h. Chlorate remaining in each sample was measured by liquid scintillation counting after [36Cl]chloride was precipitated with silver nitrate. A preliminary study indicated that chlorite, a possible intermediate in the reduction of chlorate, had a half-life of approximately 7 min in live ruminal fluid; chlorite was, therefore, not specifically measured in ruminal incubations. Chlorate dose did not affect in vitro dry matter digestion (P greater than or equal to 0.11) whereas in vitro dry matter digestibility was decreased (P less than or equal to 0.05) by 80% forage content. When data were expressed on a concentration basis (ppm), diet had no effect (P greater than or equal to 0.18) on chlorate reduction; however, when chlorate reduction was expressed on a percentage basis, chlorate reduction tended to be greater (P greater than or equal to 0.09) at h8 and 16 in incubations of the low concentrate diet. Chlorate remaining in autoclaved controls at 24 h was intermediate (P < 0.01) between chlorate remaining in “live” ruminal fluid samples incubated for 0 and 24 h. Attempts to isolate chlorate respiring bacteria from two sources of ruminal fluid were not successful. These data indicate that microbial dependent and chemical dependent reduction of chlorate occurs in bovine ruminal fluid, and that dietary concentrate had a negligible effect on chlorate reduction.