Title: Mitochondrial DNA sequences from North American Species of Lygus Authors
|Burange, Prasad - ND STATE UNIV, FARGO|
|Boetell, Mark - ND STATE UNIV, FARGO|
Submitted to: NCB-ESA North Central Branch Entomological Society of America
Publication Type: Abstract Only
Publication Acceptance Date: March 10, 2007
Publication Date: March 15, 2007
Citation: Burange, P.S., Roehrdanz, R.L., Boetell, M.A. 2007. Mitochondrial DNA sequences from North American Species of Lygus [abstract]. NCB-ESA North Central Branch Entomological Society of America. Presentation No. 029. Technical Abstract: The Lygus genus is widely distributed in North America and Eurasia. One of the most serious pest species within this genus is L. lineolaris. Inter- and intraspecific genetic diversity within the Lygus genus was investigated in North America using a part of the cox1 and cox2 genes of the mitochondrial DNA (mtDNA). A 768 bp region overlapping the cox1 and cox2 genes was amplified and sequenced. The mtDNA phylogeny indicates that most L. lineolaris individuals belong to two closely related clades. However, a few individuals were found to be distinctly divergent, and fell outside of the two primary L. lineolaris clades. Multi-species phylogenetic analysis shows that L. plagiatus, L. elisus, and L. hesperus predominantly have their own clades, although a few sequences are found on other branches[ . Some L. borealis and L. shulli also appeared to align with other species. Our results indicate that the intraspecific mtDNA variation within regional populations and between widely dispersed populations of L. lineolaris was found to be similar, thus indicating a lack of geographically based population structure for this species. The genetic diversity of several non-L. lineolaris species (i.e..e., L. hesperus, L. keltoni, L. borealis, L. elisus, L. shulli, L. plagiatus, and L. rugulipennis) collected from different geographic locations is being investigated to assess an apparent discordance between the observed gene tree and the anticipated species. This work could provide important information regarding the potential existence of host-specific or regional strains within the genus and help clarify the phylogeny of the Lygus genus.