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United States Department of Agriculture

Agricultural Research Service

Research Project: IDENTIFYING AND MANIPULATING DETERMINANTS OF PHOTOSYNTHATE PRODUCTION AND PARTITIONING Title: Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent

Authors
item Ainsworth, Elizabeth
item Gillespie, Kelly - UNIVERSITY OF ILLINOIS

Submitted to: Nature Protocols
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: February 9, 2007
Publication Date: April 12, 2007
Citation: Ainsworth, E.A., Gillespie, K.M. 2007. Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent. Nature Protocols. 2:875-877.

Interpretive Summary: Non-structural phenolic compounds provide a variety of functions to plants, including acting as antioxidants. We describe a microplate-adapted colorimetric total phenolics assay, which utilizes Folin-Ciocalteu (F-C) reagent. This assay is described with two other assays for measuring the antioxidant capacity of leaf tissue extracts. These assays provide scientists with high-throughput tools for screening the response of plants to environmental stress.

Technical Abstract: Non-structural phenolic compounds provide a variety of functions to plants, including acting as antioxidants. We describe a microplate-adapted colorimetric total phenolics assay, which utilizes Folin-Ciocalteu (F-C) reagent. The F-C assay relies on the transfer of electrons in alkaline medium from phenolic compounds to phosphomolybdic/phosphotungstic acid complexes, which are determined spectroscopically at 765 nm. While the electron transfer reaction is not specific for phenolic compounds, the described extraction procedure eliminates ~85% of ascorbic acid and other potentially interfering compounds. The assay is performed in microcentrifuge tubes and assessed in a 96-well plate reader. At least 64 samples can be processed in one day.

Last Modified: 12/18/2014
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