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United States Department of Agriculture

Agricultural Research Service

Research Project: IDENTIFYING AND MANIPULATING DETERMINANTS OF PHOTOSYNTHATE PRODUCTION AND PARTITIONING

Location: Global Change and Photosynthesis Research Unit

Title: Rapid Measurement of Total Antioxidant Capacity in Plants

Authors
item Gillespie, Kelly - UNIVERSITY OF ILLINOIS
item Chae, June
item AINSWORTH, ELIZABETH

Submitted to: Nature Protocols
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: February 9, 2007
Publication Date: April 12, 2007
Citation: Gillespie, K.M., Chae, J.M., Ainsworth, E.A. 2007. Rapid Measurement of Total Antioxidant Capacity in Plants. Nature Protocols. 2:867-870.

Interpretive Summary: There is growing interest in measuring the antioxidant status of plant tissues, in particular in response to stress. This article describes the oxygen radical absorbance capacity (ORAC) assay for measuring the total antioxidant capacity in plant tissue extracts. The high-throughput assay is performed in a microplate and is assessed with a 96-well multi-detection plate reader. This assay is presented along with rapid assays for total phenolic content and total ascorbate content. Collectively, these assays provide scientists and researchers with general diagnostic tools of the antioxidant capacity of leaf tissue extracts.

Technical Abstract: There is growing interest in measuring the antioxidant status of plant tissues. This article describes the oxygen radical absorbance capacity (ORAC) assay, which measures antioxidant inhibition of peroxyl radical-induced oxidations and is a measure of total antioxidant capacity. The assay is performed in a microplate and is assessed with a 96-well multi-detection plate reader. With the described protocol, total antioxidant capacity of 64 experimental samples can easily be analyzed in one day. This assay is presented along with rapid assays for total phenolic content and total ascorbate content. Collectively, these assays provide a general diagnostic tool of the antioxidant capacity of leaf tissue extracts.

Last Modified: 9/29/2014
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