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ARS Home » Northeast Area » Boston, Massachusetts » Jean Mayer Human Nutrition Research Center On Aging » Research » Publications at this Location » Publication #208784
Title: In vitro supplementation with white button mushroom promotes maturation of bone marrow-derived dendritic cells in mice

Author
item REN, ZHIHONG - TUFTS/HNRCA
item GUO, ZHUYAN - TUFTS/HNRCA
item Meydani, Simin
item WU, DAYONG - TUFTS/HNRCA

Submitted to: Experimental Biology
Publication Type: Abstract Only
Publication Acceptance Date: 2/6/2007
Publication Date: 4/3/2007
Citation: Ren, Z., Guo, Z., Meydani, S., Wu, D. 2007. In vitro supplementation with white button mushroom promotes maturation of bone marrow-derived dendritic cells in mice. Experimental Biology/The FASEB Journal. 21(6):p. A737.

Interpretive Summary:

Technical Abstract: Mushrooms have been shown to enhance immune response, which contributes to their anti-tumor property. White button mushrooms (Agaricus bisporus) constitute 90 percent of the total mushroom market in the US; however, the health benefit of this strain in general is not well-studied. Furthermore, little is known about its immunologic effects. We have recently found that dietary supplementation with white mushroom enhances natural killer (NK) cell activity in mice. Dendritic cells (DC) are most potent antigen presenting cells and play a pivotal role in immune response by linking innate and adaptive immune responses. DC also play a predominant role in activation of NK cells and thus are directly involved in innate immunity. In this study we investigated the effect of in vitro supplementation with white mushrooms on maturation of bone marrow-derived DC (BMDC) of C57BL mice. BMDC were differentiated by addition of GM-CSF and IL-4 in the presence of solution of whole mushroom extract at 50, 100, or 200 microg/ml. Results showed that mushroom supplementation dose-dependently increased the expression of maturation markers CD40, CD80, CD86 and MHC-II as indicated by both the frequency of cells positive for the above markers and their intensity per cell. Consistent with the changes in the phenotypic makers, functional assay for DC maturation showed that mushroom supplementation induced a decrease in endocytosis and an increase in intracellular IL-12 levels of DC. In conclusion, white button mushroom promotes DC maturation and this effect may have a potential in enhancing both innate and T cell-mediated immunity, which play a key role in body’s anti-microbial and anti-tumor defense. Supported by USDA #58-1950-9-001 and a grant from the Mushroom Council, San Jose, CA.