Submitted to: Journal of Liquid Chromatography and Related Technologies
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: April 24, 2007
Publication Date: June 20, 2007
Citation: Lin, J.T. 2007. Hplc separation of acyl lipid classes. Journal of Liquid Chromatography and Related Technologies. 30: 2005-2020. Interpretive Summary: High-performance liquid chromatography (HPLC) for the separation of various lipid classes is reviewed here. The identification of the molecular species of acyl lipids in a biological sample requires steps of lipid extraction, separation of lipid classes, separation of the molecular species of a lipid class, and mass spectrometry. Separation of lipid classes is one of the important steps in lipid identification and quantification. Lipid analysis is an important part of our research here.
Technical Abstract: Identification of complex acyl lipids ideally includes normal-phase HPLC to separate the acyl lipid classes followed by reversed-phase HPLC to separate the molecular species of a lipid class. Both polar lipid classes and non-polar lipid classes have been separated by normal-phase HPLC, mostly on silica and diol columns using binary gradients. The tentative identification of lipid classes can be made by HPLC retention times and the cochromatography with lipid class standards. Various normal-phase HPLC systems separating lipid classes are reviewed here.