|Bosques, Joanel - UNIV PUERTO RICO-MAYAGUEZ|
|Pagan, Melvin - UNIV PUERTO RICO-MAYAGUEZ|
Submitted to: Journal of Animal Science Supplement
Publication Type: Abstract Only
Publication Acceptance Date: February 15, 2007
Publication Date: July 8, 2007
Repository URL: http://adsa.psa.ampa.asas.org/meetings/2007/abstracts/0188.PDF
Citation: Bosques, J.H., Pagan, M., Casas, E. 2007. Segregation of polymorphisms at Calpain and Calpastatin in beef cattle grown in the tropics [abstract]. Journal of Animal Science. 85(Suppl. 1):191. Abstract #219. Technical Abstract: The distribution of two single nucleotide polymorphisms (SNP) in the CAPN1 gene (CAPN1-316 and CAPN1-530) and in Calpastatin (CAST) was determined in beef cattle grown in the tropics (n = 372). Genotypic and allelic frequencies were determined in Senepol (n=60), Charolais (n=62), Angus (n=39), Charbray (n=43), Brahman (n=19), Zebu (n=17) and crossbred bulls (n=132). For CAPN1-316, genotypic frequencies were 0.05, 0.63, and 0.32, for AA, AG, and AG genotypes, respectively. Allele frequencies were 0.21 and 0.79 for A and G alleles. The AA genotype was absent in Charbray, Charolais, Angus, Zebu and Brahman breeds. In Charolais and Senepol GG was greater than AG and the inverse was observed for Zebu, Brahman and Crossbred bulls. In Angus, the AG and GG genotypes were evenly distributed. The A allele was more frequent in Charolais, Angus, Zebu and Brahman, whereas the G allele predominated in Charbray and crossbred animals. Genotypic frequencies for the SNP in CAPN1-530 region were 0.17, 0.40, and 0.43 for CC, CT, and TT genotypes respectively. Allelic frequencies were 0.39 and 0.61 for C and T. Animals of CT genotype were more frequent in Charolais, Senepol, Angus and crossbred, whereas TT was more common in Charbray, Zebu and Brahman bulls. Greater allelic frequencies for C were observed in Senepol and Brahman. The inverse was observed in the other breeds. For CAST, genotypic frequencies were 0.03 (n=10), 0.26 (n=79), and 0.71 (n=79), for CC, CT, and TT, respectively. Overall allele frequencies were 0.46/A and 0.54/C. The allelic frequency of C was greater in Charolais and Angus bulls. Animals having CC genotype were absent in Charolais, Angus and Brahman animals genotyped. The TT animals were more frequent in Charbray, Charolais, Senepol, Angus Brahman, Zebu and crossbred animals. The segregation of the polymorphisms in CAPN1 and CAST could be implicated in differences observed in economically important traits in beef cattle grown in tropical environments.