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United States Department of Agriculture

Agricultural Research Service

Title: Effect of Time and Sand Abrasion on Recovery of Aerobic Bacteria, Escherichia coli, and Coliforms from Broiler Carcasses

Authors
item Hannah, J - UGA POULTRY SCI
item Cox, Nelson
item Smith, Douglas
item Cason Jr, John
item Fletcher, D - UGA DEPT OF ANML SCI
item Northcutt, Julie
item Buhr, Richard
item Richardson, Larry

Submitted to: Poultry Science Association Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: February 27, 2007
Publication Date: July 8, 2007
Citation: Hannah, J.F., Cox Jr, N.A., Smith, D.P., Cason Jr, J.A., Fletcher, D.L., Northcutt, J.K., Buhr, R.J., Richardson, L.J. 2007. Effect of Time and Sand Abrasion on Recovery of Aerobic Bacteria, Escherichia coli, and Coliforms from Broiler Carcasses. Poultry Science Association Meeting Abstract.8 86:(Suppl. 1)442, P.384.

Technical Abstract: An experiment was conducted to determine the effect of rinse time and a sand abrasion on bacteria from whole broiler carcass rinses (WCR). Twelve eviscerated broiler carcasses were obtained from a commercial processing plant prior to chilling. Six carcasses were rinsed in 400 mL of 2.0% buffered peptone for 1 and 4 min utilizing a mechanical shaker. The remaining carcasses were rinsed in the same manner, but with 100 g of sterile sand added to the rinse solution. Rinses were analyzed for aerobic bacteria (APC), Escherichia coli, and coliforms. For APC, E. coli and coliforms, the level recovered from the sand rinses were significantly higher (P<0.05) than the level recovered from peptone rinses. APC, E. coli and coliforms collected from the peptone rinses were 4.0, 3.1, and 3.4 log10 cfu/mL of rinse, respectively. APC, E. coli and coliforms collected from the sand rinses were 4.6, 3.7, and 4.1 log10 cfu/mL of rinse, respectively. There was no significant difference in bacterial recovery from the two rinse times for either treatment (P>0.05). A secondary determination of treatment efficacy was conducted by swabbing a 25cm2 area of breast skin before and after rinsing. Swabs from carcasses rinsed with sand yielded lower APC, but the difference was not significant. In this study, utilizing a sand abrasion during WCR had a significant increase on bacterial recovery and rinsing time was found not to be significant on recovery. In addition, WCR was a more sensitive method for recovery of bacteria than swabbing.

Last Modified: 8/1/2014
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