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United States Department of Agriculture

Agricultural Research Service

Research Project: MINIMIZING THE ADVERSE HEALTH AND ECONOMIC IMPACTS OF MYCOTOXINS AND PLANT TOXINS IN FOODS Title: Effect of extrusion cooking, with and without added glucose, on fumonisin B1 in corn grits.

Authors
item Voss, Kenneth
item Jackson, L - NCFST/FDA,ARGO-SUM., IL
item Jablonski, J - MCFST/FDA,ARGO-SUM., IL
item Bianchini, A - FOOD SCI/TECH,UNE,LINCOLN
item Bullerman, L - FOOD SCI/TECH,UNE,LINCOLN
item Hanna, M - FOOD SCI/TECH,UNE,LINCOLN
item Rye, D - FOOD SCI/NUTR,TX WOMANS U

Submitted to: Meeting, Center for Food Safety, University of Georgia
Publication Type: Abstract Only
Publication Acceptance Date: January 30, 2007
Publication Date: February 28, 2007
Citation: Voss, K.A., Jackson, L.S., Jablonski, J., Bianchini, A., Bullerman, L.B., Hanna, M.A., Rye, D. 2007. Effect of extrusion cooking, with and without added glucose, on fumonisin B1 in corn grits. university of Georgia's Center for Food Safety. February 28,2007. Atlanta,GA.

Interpretive Summary: Abstract - no summary required.

Technical Abstract: Extrusion cooking reduces fumonisin concentrations in corn but how it affects fumonisin toxicity is not well characterized. A batch of corn grits (SG) was spiked with fumonisin B1 (FB1) and two batches (FG1 & FG2) were contaminated with FB1 by fermentation. Respective FB1 concentrations of SG, FG1 and FG2 were 43, 46, and 67 nmol/g (dry weight). SG, FG1 and FG2 were then extruded both with and without glucose supplementation (10% w/w). FB1 concentrations of the extruded products E-SG, E-FG1, and E-FG2 were 34 (21% reduction), 29 (37% reduction), and 51 nmol/g (24% reduction). Supplementation with glucose further decreased FB1 concentrations of the cooked materials: respective FB1 concentrations of E-SG plus glucose, E-FG1 plus glucose and E-FG2 plus glucose extrusion products were 9.8 (77% reduction), 5.9 (87% reduction), and 12 nmol/g (83% reduction). Significant amounts (20-38 nmol/g) of the FB1-glucose reaction product, N-(deoxy-D-fructos-1-yl) FB1 were found only in the glucose-supplemented products. For toxicity studies, SG, FG1 or FG2 (50% w/w) and equivalent weights of each extruded product were fed to male rats for three weeks. Two control groups were fed (50% w/w) uncooked or extruded uncontaminated grits. Relative kidney weights of rats fed E-FG1 plus glucose (0.83%) were similar to control values (0.79-0.82%) and greater than those found in all other test groups (0.72-0.76%). Histopathology revealed the presence of kidney lesions consistent with FB1 exposure in all groups except the controls. Lesions found in rats fed E-FG1 plus glucose were less severe than those from the FG1 or E-FG1 groups. However, extrusion with or without glucose supplementation did not affect the severity of kidney lesions induced by SG or FG2. Together, these findings confirmed that extrusion with glucose supplementation significantly reduces the amount of FB1 in contaminated corn grits (>75%) but that more studies are needed to determine its affect on toxicity.

Last Modified: 12/18/2014
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