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United States Department of Agriculture

Agricultural Research Service

Research Project: DEVELOPMENT OF AN INTEGRATED RISK MODEL FOR FOODBORNE ZOONOTIC PARASITES IN SWINE Title: Isolation and genetic characterization of toxoplasma gondii from striped dolphin (Stenella coeruleoalba) from Costa Rica

Authors
item Dubey, Jitender
item Morales, J - COSTA RICA
item Sundar, N - USDA ARS BELTSVILLE MD
item Velmurugan, G - USDA ARS BELTSVILLE MD
item Gonzalez, Barrientos - COSTA RICA
item Hernandez-Mora, G - COSTA RICA
item Su, C - J TENNESSEE, KNOXVILLE

Submitted to: Journal of Parasitology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: February 23, 2007
Publication Date: May 1, 2007
Citation: Dubey, J.P., Morales, J.A., Sundar, N., Velmurugan, G.V., Gonzalez, B.C., Hernandez-Mora, G., Su, C. 2007. Isolation and genetic characterization of Toxoplasma gondii from striped dolphin (Stenella coeruleoalba) from Costa Rica. Journal of Parasitology. 93:709-710.

Interpretive Summary: Toxoplasma gondii is a single-celled parasite of all warm-blooded hosts worldwide. It causes mental retardation and loss of vision in children, and abortion in livestock. Cats are the main reservoir of T. gondii because they are the only hosts that can excrete the resistant stage (oocyst) of the parasite in the feces. Humans become infected by eating undercooked meat from infected animals and food and water contaminated with oocysts.Toxoplasmosis causes mortality in many species of animals in the zoos, especially primates. Scientists at the Beltsville Agricultural Research Center and a University in Costa Rica report first genetic characterization of Toxoplasma from a dolphin. The results will be of interest to biologists, parasitologists, and veterinarians

Technical Abstract: Toxoplasma gondii infection in marine mammals is of interest because of mortality and mode of transmission. It has been suggested that marine mammals become infected with T. gondii oocysts washed from land to the sea. We report the isolation and genetic characterization of viable T. gondii from a striped dolphin (Stenella coeruleoalba), the first time from this host. An adult female dolphin was found stranded on the Pacific Coast of Costa Rica, and the animal died the next day. The dolphin had a high (1:6,400) antibody titer to T. gondii in the modified agglutination test. Severe nonsuppurative meningo-encephalomyelitis was found in its brain and spinal cord, but T. gondii was not found in histological sections of the dolphin. Portions of its brain and the heart were bioassayed in mice for the isolation of T. gondii. Viable T. gondii was isolated from the brain, but not from the heart, of the dolphin. A cat fed mice infected with the dolphin isolate (designated TgSdCo1) shed oocysts. Genomic DNA from tachyzoites of this isolate was used for genotyping at 10 genetic loci, including SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico, and this TgSdCo1 isolate was found to be Type II.

Last Modified: 7/22/2014