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United States Department of Agriculture

Agricultural Research Service

Title: Effect of hydrodynamic pressure processing and aging on sarcoplasmic proteins of beef strip loins

Authors
item BOWKER, BRIAN
item Fahrenholz, Timothy
item Paroczay, Ernest
item Solomon, Morse

Submitted to: Journal of Muscle Foods
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: February 15, 2007
Publication Date: April 2, 2008
Citation: Bowker, B.C., Fahrenholz, T.M., Paroczay, E.W., Solomon, M.B. 2008. Effect of hydrodynamic pressure processing and aging on sarcoplasmic proteins of beef strip loins. Journal of Muscle Foods. 19:175-193.

Interpretive Summary: Evaluating the effects of hydrodynamic pressure processing (HDP) and aging on fresh beef strip loins, this study found that both HDP and aging caused changes to the composition and solubility of the sarcoplasmic protein component of muscle. Data suggest that sarcoplasmic protein changes may be useful indicators of the proteolysis related to muscle tenderization.

Technical Abstract: This study evaluated the effects of hydrodynamic pressure processing (HDP) and aging on the sarcoplasmic proteins of beef strip loins. Loins (n=12) were halved at 48 h postmortem and assigned to HDP or control treatments. Following treatment, each half was divided into three portions for aging (0, 5, or 8 days). Samples were removed for Warner-Bratzler shear force (WBSF) determination and sarcoplasmic protein isolation. SDS-PAGE analysis found that HDP influenced protein bands corresponding to 143, 65, 44, 36, and 19 kDa. Aging influenced the band intensity of 22 sarcoplasmic proteins. Changes in sarcoplasmic protein composition were significantly correlated to WBSF (r= -0.58 to 0.45). Sarcoplasmic protein solubility decreased with both HDP (P<0.05) and aging (P<0.0001). Changes in solubility were significantly correlated to SDS-PAGE band intensities (r= -0.53 to 0.60). Data suggest that HDP and aging cause changes to sarcoplasmic proteins that may be indicators of proteolysis and tenderization.

Last Modified: 9/10/2014
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