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United States Department of Agriculture

Agricultural Research Service

Research Project: MICROBIAL MODELING AND BIOINFORMATICS FOR FOOD SAFETY AND SECURITY Title: Comparison of virulence plasmid (pYV/pCD)-associated phenotypes in Yersinia species

Author
item Bhaduri, Saumya

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: January 20, 2007
Publication Date: July 8, 2007
Citation: Bhaduri, S. 2007. Comparison of virulence plasmid (pYV/pCD)-associated phenotypes in Yersinia species. Abstract. International Associatin for Food Protection. T5-11.

Technical Abstract: Phenotypic expression of several virulence plasmid (pYV/pCD; 70-kb)-associated genetic determinants were compared among Yesinia species; Yesinia pestis, (an attenuated strain which does not cause plague designated to be used in BSL2 facility), Y. pseudotuberculosis and Y. enterocolitica including low calcium response (Lcr: pin point colony; size: 0.36 mm), colony morphology (size: 1.13 mm), crystal violet (CV) binding (dark-violet colony), Congo Red (CR) uptake (red : pin point colony; size: 0.36 mm), autoagglutination (AA: cells agglutinate), and hydrophobicity (HP: forms clumps.) All three species demonstrated Lcr, CV binding, and CR-uptake. However, CR-uptake in Y. pestis was demonstrated only on calcium-deficient CR magnesium oxalate tryptic soy agar (CRMOX), whereas this phenotype was expressed on both CRMOX and low-calcium agarose media for Y. pseudotuberculosis and Y. enterocolitica. These phenotypes were detectable within 24 h at 37 degree C in Y. enterocolitica and Y. pseudotuberculosis, but at 48 h in Y. pestis due to slower growth rate at 37 degree C. The colony morphology/size, AA, and HP characteristics were expressed in Y. pseudotuberculosis as in Y. enterocolitica, but these three phenotypes were not expressed in Y. pestis. Moreover, CR-uptake by Y. pestis in the calcium deficient medium and differences in time to express Lcr, CR-uptake, and CV binding provides the means to identify Y. pestis colonies in clinical samples, animals, and food.

Last Modified: 12/19/2014
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