ARS | Publication request: Effect of Biotic Elicitors on Enrichment of Antioxidant Properties and Induced Isoflavones in Soybean

Submitted to: Journal of Food Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: January 28, 2008
Publication Date: March 29, 2008
Citation: Boue, S.M., Shih, F.F., Shih, B.Y., Daigle, K.W., Carter Wientjes, C.H., Cleveland, T.E. 2008. Effect of Biotic Elicitors on Enrichment of Antioxidant Properties and Induced Isoflavones in Soybean. Journal of Food Science. 73:H43-H49.

Interpretive Summary: The antioxidant activities of seed extracts from three soybean varieties derived before/after germination and with/without the use of elicitors were compared. The isoflavone content was determined for each seed extract. Antioxidant activities of the extracts were measured using two different methods. Higher antioxidant activities were observed in wounded and elicitor-treated extracts when compared with non-wounded control extracts. In addition, the phenolic content was higher in extracts from wounded and elicitor-treated soybean. Soybean variety Asgrow 5902 had the highest total isoflavone content of the three varieties tested. Elicitor treated soybean extracts from Asgrow 5902 variety had the highest total isoflavone content and the highest antioxidant activities in both antioxidant assays. Also identified in the wounded and elicitor treated extracts were the phytoalexin glyceollins.

Technical Abstract: The antioxidant activities of seed extracts from three soybean varieties derived before/after germination and with/without the use of elicitors were compared. Isoflavone content was determined by C18 reverse phase high-performance chromatography coupled with a photodiode array detector. Antioxidant activities of the extracts were measured using 2,3-diphenyl-1-picryl-hydrazyl (DPPH) free radical scavenging and B-carotene co-oxidation in a linoleate system. Higher antioxidant activities were observed in wounded and elicitor-treated extracts when compared with non-wounded control extracts. In addition, the phenolic content was higher in extracts from wounded and elicitor-treated soybean. Soybean variety Asgrow 5902 had the highest total isoflavone content of the three varieties tested. Elicitor treated soybean extracts from Asgrow 5902 variety had the highest total isoflavone content and the highest antioxidant activities in both the DPPH and B-carotene assays. Also identified in the wounded and elicitor treated extracts were the phytoalexin glyceollins.