|Buboltz, Anne - PENNSYLVANIA STATE UNIV|
|Harvill, Eric - PENNSYLVANIA STATE UNIV|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: September 1, 2006
Publication Date: November 7, 2006
Citation: Buboltz, A., Nicholson, T.L., Harvill, E. 2006. Loss of Adenylate Cyclase Tonxin among closely related B. bronchiseptica strains [abstract]. Eighth International Symposium: Saga of the Genus Bordetella, November 7-10, 2006, Pasteur, Paris. p. 26. Technical Abstract: Bordetella bronchiseptica is a multi-host, gram-negative respiratory pathogen that causes everything from asymptomatic infection to fatal pneumonia. We have identified a strain of B. bronchiseptica, 253, that is inefficient at persisting in the lower respiratory tract of mice compared to the typically studied strain, RB50. Strain 253 lacks expression of Adenylate Cyclase Toxin (ACT). Comparative Genomic Hybridization microarray and PCR analysis suggest that genes required for ACT expression and function (cyaA, cyaB and cyaC) are either greatly mutated or deleted. Interestingly, other strains of the same multi-locus sequence type (MLST) also lack ACT expression. This data indicates that the lack of ACT expression is correlated with a inefficient persistence in the lower respiratory tract of mice. Combined, these data suggest that even though ACT expression is lacking in these closely related strains, they are epidemiologically successful.