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United States Department of Agriculture

Agricultural Research Service

Title: A SolCAP strategy to evaluate allelic diversity of cultivated and wild Solanum germplasm and access economically important genes

Authors
item Douches, David - MICHIGAN STATE UNIVERSITY
item DE Jong, Walter - CORNELL UNIVERSITY
item Jansky, Shelley
item Spooner, David
item Brown, Charles
item Francis, David - OHIO STATE UNIVERSITY

Submitted to: American Journal of Potato Research
Publication Type: Abstract Only
Publication Acceptance Date: March 15, 2006
Publication Date: N/A

Technical Abstract: THE Solanaceae Coordinated Agricultural Project (SolCAP) is an effort to organize a community of public and private researchers for “translational genomics”, the utilization of information derived from genome technologies for crop improvement. Standard germplasm panels will be developed that represent a balance between relevant breeding lines and populations for association mapping of relevant traits or marker-assisted-selection. The germplasm panel will be grouped according to specific market classes and will include a broad genetic base to survey the allelic diversity for important traits, develop pertinent markers and test candidate gene function. A subset of the panel will be used for deep-sequencing of genes and promoters related to carbohydrate metabolism. Another set will be important varieties and advanced lines of value to the breeding community. To provide a taxonomic perspective, a set of Solanum species and accessions will be sampled representing all major clades, ploidies, and breeding systems. Lastly, two mapping populations (2x and 4x) will be chosen for genotyping. With a well-designed panel, we should also be able to examine domestication events (i.e. genes selected during domestication in wild to cultivated transitions zones). SSR and SNP marker platforms will be used to genotype the entire germplasm panel. Adopting standardized genotyping platforms and developing user-friendly tools for analysis will help translate genome sequence information and increase access to the breeding community. At the same time such approaches will help elucidate the genes and gene systems under selection during domestication and breeding.

Last Modified: 11/24/2014